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nonimmune rabbit serum  (Agilent technologies)


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    Structured Review

    Agilent technologies nonimmune rabbit serum
    Nonimmune Rabbit Serum, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonimmune+rabbit+serum/pmc08788708-130-10-13
    Average 90 stars, based on 1 article reviews
    nonimmune rabbit serum - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstr?m macroglobulinemia
    Article Snippet: Anti-BLyS (R & D Systems) and nonimmune rabbit serum (10 μg/mL; DakoCytomation California, Carpinteria, CA) were added to slides for 30 minutes.

    Article Title: Immunohistochemical detection of receptor activator nuclear κB ligand and osteoprotegerin in odontogenic cysts and tumors.
    Article Snippet: Odontogenic cysts and tumors constitute a heterogeneous group of jaw lesions with diverse histopathologic features and clinical manifestations.. Bone resorption is a major biologic event responsible for the progression and aggressiveness of these lesions and depends on the formation and activation of osteoclasts.. The expression of factors stimulating and inhibiting bone resorption has been correlated with the development of these lesions, with emphasis on the osteoprotegerin (OPG) known as osteoclastogenesis inhibitory factor and receptor activator

    Article Title: Role of Renal Sympathetic Nerve Activity in Volatile Anesthesia's Effect on Renal Excretory Function
    Article Snippet: As negative controls, the primary antibody was substituted with nonimmune rabbit serum (X0936, DAKO Sweden AB, Stockholm, Sweden).

    Article Title: Suppression of proliferation, tumorigenicity and metastasis of lung cancer cells after their transduction by interferon-beta gene in baculovirus vector.
    Article Snippet: http://dx.doi.org/10.1016/j.cyto.2014.10.029 1043-4666/ 2014 Elsevier Ltd. Abbreviations: IFN-beta, interferon-beta; BV, baculovirus vector; rBV, recombinant baculovirus vector without interferon-beta gene; rBV/IFN-beta, recombinant baculovirus vector with interferon-beta gene; plaque forming unit, pfu; NCS, newborn calf serum; MOI, multiplicity of infection; VSV, vesicular stomatitis virus; colony forming activity, CFA; IU, international units; i/m, intramuscularly; MHC, major histocompatibility complex; DCs, dendritic cells; AcMNPV, Autographa californica multiple nuclear polyhedrosis virus; PBS, phosphate buffered saline; EMT, epithelial–mesenchymal transition; TLR, Toll-like receptor; MSCs, mesenchymal stem cells.. ⇑ Corresponding author.

    Control:

    Article Title: Sustained hypoglycemia affects glucose transporter expression of human blood leukocytes.
    Article Snippet: The scarce data available on leukocyte glucose transporter expression are contradictory and nothing is known about its regulation by glycemic state.. Therefore, cytospin preparations of blood leukocytes were searched immunocytochemically for the high-affinity glucose transporters GLUT1, 3, and 4.. Hypoglycemia-associated quantitative changes in transporter expression were assessed by flow cytometry.

    Incubation:

    Article Title: Sustained hypoglycemia affects glucose transporter expression of human blood leukocytes.
    Article Snippet: The scarce data available on leukocyte glucose transporter expression are contradictory and nothing is known about its regulation by glycemic state.. Therefore, cytospin preparations of blood leukocytes were searched immunocytochemically for the high-affinity glucose transporters GLUT1, 3, and 4.. Hypoglycemia-associated quantitative changes in transporter expression were assessed by flow cytometry.

    Negative Control:

    Article Title: Expression of bone resorption regulators (RANK, RANKL, and OPG) in odontogenic tumors.
    Article Snippet: Objective.. To investigate the expression of bone resorption regulators (receptor activator of nuclear factor kappa B [RANK], RANK ligand [RANKL], and osteoprotegerin [OPG]) in calcifying cystic odontogenic tumor (CCOT), adenomatoid odontogenic tumor (AOT), calcifying epithelial odontogenic tumor (CEOT), odontogenic myxoma (OM), and ameloblastic fibroma (AF).. Study design.



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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Journal: American Journal of Cancer Research

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    doi:

    Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

    Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR